Hitachi S-4700 Cold Field Emission Gun
Description:
A Cold Field Emission Gun Scanning Electron Microscope (FEGSEM) of "below‑the‑lens" design capable of (manufacturer's claims) 1.5 nm resolution at 15 kV, 12 mm W.D.; and 2.5 nm resolution at 1 k, 2.5 mm W.D. Magnification ranges from 30X to 500,000X. Specimen tilt at 12 mm W.D. up to 45 degrees. Electron source is a cold FE gun producing high brightness (~ 2 x 109 A / cm2/sr) with little energy spread (0.2 - 0.3 eV). The "below‑the‑lens" design and large sample chamber port permits samples as large as 100 mm diameter x 17 mm thick. Oil-free vacuum systems pump column and sample exchange. Available image modes include secondary and backscattered electron images. There are two secondary electron detectors; one above the objective lens, the other below. Digital images may be acquired in BMP, TIFF, or JPEG file formats at 640 x 480, 1280 x 960, or 1560 x 1920 pixels.
Applications:
- High-resolution topographic contrast (Se) and atomic number contrast (BSe) imaging of biological and non-biological samples in both room temperature and cryo modes
- Applicable to imaging of immunogold labeled individual cells and bacteria
Specifications:
- High-resolution topographic contrast (Se) and atomic number contrast (BSe) imaging of biological and non-biological samples in both room temperature and cryo modes
- Cold field-emission gun
- Resolution: 1.5 nm at 15 kV, 12 mm W.D.; and 2.5 nm resolution at 1 k, 2.5 mm W.D.
- Magnification ranges from 30X to 500,000X.
- Two secondary electron detectors: one above the objective lens, the other below
- Backscatter imaging at TV rates and low voltage (threshold 2.5 kV on gold) with Autrata modified YAG (yttrium aluminum garnet, cerium doped) crystal
- Sample size up to 100 mm diameter x 17 mm high
- Specimen tilt at 12 mm W.D up to 45 degrees.
- Digital image acquisition at 640 X 480, 1280 X 960, or 1560 X 1920 pixels
Equipment/Accessories:
- Hitachi S-4700 with a variety of non-cryo sample holders
- CryoSEM observation using Emitech Cryo Stage, Model K-1250 Cryopreparation System, and a variety of sample holders
- Backscatter imaging at TV rates and low voltage (threshold 2.5 kV on gold) with Autrata modified YAG (yttrium aluminum garnet, cerium doped) crystal
- Keithly Specimen Current Detector
Capabilities:
| Specimen Stage, Non-Cryo |
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| Specimen Stage, Cryo |
Traverses similar to above, but rotation limited to 30 degrees clockwise, and 180 degrees counterclockwise, from zero point. |
