The goal of my research is to develop new biomedical technologies for systems-level investigation of RNA regulation in living tissues and organisms. Using the novel techniques, I am interested in understanding how gene expression is controlled at the RNA level in the neural networks. One of the fundamental questions in neuroscience is how neural activities modulate the connectivity. Activity-dependent transcription and local protein synthesis are critical events involved in the formation and alteration of neural circuits. However, it is still unclear how de novo gene expression occurs in response to neural activities in real time at the single-cell, single-molecule level.
In my laboratory, we are studying the dynamics of transcription and RNA localization in live cells and tissues. Using our unique mouse models where every β-actin or Arc mRNA is fluorescently labeled, we have demonstrated the first single-molecule imaging of endogenous mRNA in live neurons and brain tissues. We have also developed new image analysis tools and theoretical models for mRNA transport in dendrites. These achievements will be the foundation for my long-term pursuit of non-invasive quantitative interrogation of gene expression in live animals and humans. My research is based on a multifaceted systems approach combining genetic engineering, RNA technology, in vivo imaging, and quantitative single-molecule analysis.
Ph.D., Applied Physics, 2007, Cornell University, Ithaca, NY, United States M.S., Applied Physics, 2001, Cornell University, Ithaca, NY, United States B.S., Physics, 1999, Seoul National University, Seoul, Republic of Korea
2019 Early Career Award, Federation of American Societies for Experimental Biology (FASEB) 2017 HHMI-Wellcome International Research Scholar Award, Howard Hughes Medical Institute (HHMI) and Wellcome Trust 2017 Excellent Lecture Award, Seoul National University 2015 Chung-Am Science Young Investigator Fellowship, POSCO Chung-Am Foundation 2009 Ruth L. Kirschstein National Research Service Awards for Postdoctoral Fellows (F32), NIH 2000 W. M. Keck Fellowship, Cornell University 1999 Sage Fellowship, Cornell University
Ahn, H., Durang, X., Shim, J. Y.,Park, G., Jeon, J.-H†, and Park, H. Y.†. (2023). Statistical Modeling of mRNP Transport in Dendrites: A Comparative Analysis of β-actin and Arc mRNP Dynamics. Traffic 24, 522. (*co-first authors; †co-corresponding authors).
Noh, K., Cho, W.-H., Lee, B. H., Kim, D. W., Kim, Y. S., Park, K., Hwang, M., Barcelon, E., Cho, Y. K., Lee, C. J., Yoon, B.-E., Choi, S.-Y., Park, H. Y., Jun, S. B., and Lee, S. J. (2023). Cortical Astrocytes Modulate Dominance Behavior in Male Mice by Regulating Synaptic Excitatory and Inhibitory Balance. Nature Neuroscience 26, 1541.
Lee, C.*, Lee, B. H.*, Jung, H.*, Lee, C., Sung, Y., Kim, H., Shim, J. Y., Kim, J., Choi, D. I., Park, H. Y.†, and Kaang, B.-K.† (2023). Hippocampal Engram Networks for Fear Memory Recruit New Synapses and Modify Pre-existing Synapses in vivo. Current Biology 33, 507 (*co-first authors; †co-corresponding authors).
Durang, X.†, Ahn, H.,Shim, J. Y.,Park, H. Y. †, and Jeon, J.-H†. (2023). Accessing Power-Law Statistics Under Experimental Constraints. Physical Review Research 5, 013011 (†co-corresponding authors).
Lee, B. H., Shim, J. Y., Moon, H. C., Kim, D. W., Kim, J., Yook, J. S., Kim, J., Park, H. Y. (2022). Real-Time Visualization of mRNA Synthesis During Memory Formation in Live Mice. Proceedings of the National Academy of Sciences 119, e2117076119.
Park, S. Y., Moon, H. C., and Park, H. Y. (2020). Live-Cell Imaging of Single mRNA Dynamics using Split Superfolder Green Fluorescent Proteins with Minimal Background. RNA 26, 101-109.
Das, S., Moon, H. C., Singer, R. H., and Park, H. Y. (2018). A Transgenic Mouse for Imaging Activity-Dependent Dynamics of Endogenous Arc mRNA in Live Neurons. Science Advances 4, eaar3448.
Song, M. S., Moon, H. C., Jeon, J.-H., and Park, H. Y. (2018). Neuronal Messenger Ribonucleoprotein Transport Follows an Aging Lévy Walk. Nature Communications 9, 344.
Lee, B. H. and Park, H. Y. (2018). HybTrack: A Hybrid Single Particle Tracking Software Using Manual and Automatic Detection of Dim Signals. Scientific Reports 8, 212.
Vera, M., Biswas, J., Senecal, A., Singer, R. H., and Park, H. Y. (2016). Single Cell and Single-Molecule Analysis of Gene Expression Regulation. Annual Review of Genetics 50, 267.
Park, H. Y., Lim, H., Yoon, Y. J., Follenzi, A., Nwokafor, C., Lopez-Jones, M., Meng, X., and Singer, R. H. (2014). Visualization of Dynamics of Single Endogenous mRNA Labeled in Live Mouse. Science 343, 422.
Park, H. Y., Kim, S. A., Korlach, J., Rhoades, E., Kwok, L. W., Zipfel, W. R., Waxham, M. N., Webb, W. W., and Pollack, L. (2008). Conformational Changes of Calmodulin upon Ca2+ binding studied with a Microfluidic Mixer. Proceedings of the National Academy of Sciences of the USA 105, 542.