Vincent Noireaux

man wearing grey flannel

Vincent Noireaux

Professor, School of Physics and Astronomy


Physics And Nanotechnology Building
Room 416
115 Union St. Se
Minneapolis, MN 55455


Noireaux Lab


Ph.D., University Paris XI (France), 2000 

B.S., Tours University (France), 1994

Professional Background

Postdoc Biological Physics, The Rockefeller University, New York, 2000-2005 
In vitro gene expression, synthetic gene circuits and synthetic cell system.

PhD: movement of the bacteria Listeria monocytogenes, a model of cell motility. A biomimetic system was used to understand actin-based motility in living cells.

Research Interests

Experimental biological physics and synthetic biology. In vitro gene expression, development of quantitative cell-free transcription/translation systems, biophysics of a synthetic cell system.

Selected Publications

Noireaux, V., Liu, A.P. The new age of cell-free biology. Annu. Rev. Biomed. 22, 51-77 (2020)

Garenne, D., Libchaber, A.J., Noireaux, V., Membrane molecular crowding enhances MreB polymerization to shape synthetic cells from spheres to rods. Proc. Nat. Acad. Sci. USA 117, 1902-1909 (2020)

Agrawal, .K., Marshall, R., Noireaux, V., Sontag, E.D. In vitro implementation of robust gene regulation in a synthetic biomolecular integral controller. Nat Commun 10, 5760 (2019)

Marshall, R. Beisel, C.L., Noireaux, V. A detailed protocol to rapidly test CRISPR nucleases and guide RNAs in an E. coli Cell-Free Transcription-Translation System. STAR Protocols 1, (2019)

Marshall, R., Noireaux, V. Quantitative modeling of transcription and translation of an all E. coli cell-free system. Sci Rep 9(1), 11980 (2019)

Garamella, J., Majumder, S., Liu, A.P., Noireaux, V. An adaptive synthetic cell based on mechanosensing, biosensing, and inducible gene circuits. ACS Synth Biol, 8(8), 1913-1920 (2019)

Yim, S.S., Johns, N.I, Park, J., Gomes, A.L.C., McBee, R.M., Richardson, M., Ronda, C., Chen, S.P., Garenne, D., Noireaux, V., Wang, H.H. Multiplex transcriptional characterizations across diverse bacterial species using cell-free systems. Mol System Biol, 15(8), e8875 (2019)

Izri, Z., Garenne, D., Noireaux, V., Maeda Y.T. Bimodal gene expression in on-chip membrane-bound artificial cells. ACS Synth Biol, 8(8), 1705-1712 (2019)

Garenne, D., Noireaux, V. Cell-free transcription-translation: engineering biology from the nanometer to the millimeter scale. Curr Opin Biotechnol 58:19-27 (2018)

Sakamoto, R., Noireaux, V., Maeda, Y. Anomalous scaling of gene expression in confined cell-free reactions. Sci Rep 8(1), 7364 (2018)

Condon, A., Kirschner, H., Lariviere, D., Marshall, W., Noireaux, V., Tlusty, T., Fourmentin, E. Will biologists become computer scientists? EMBO Rep 19(9), e46628 (2018)

Marshall, R., Noireaux, V. Synthetic biology with an all E. coli TXTL system: quantitative characterization of regulatory elements and gene circuits. Methods Mol Biol 1772: 61-93 (2018)

Rustad, M., Eastlund, A., Jardine, P., Noireaux, V. Cell-free TXTL synthesis of infectious bacteriophage T4 in a single test tube reaction. Synthetic Biology 3(1) ysy002 (2018)

Klocke, M.A., Garamella, J., Subramanian, H.K.K., Noireaux, V., Franco, E. Engineering DNA nanotubes for resilience in an E. coli TXTL system. Synthetic Biology 3(1) ysy001 (2018)

Marshall, R., Maxwell, C.S., Collins, S.P., Jacobsen, T., Luo, M.L., Begemann, M.B., Gray, B.N., January, E., Singer, A., He, Y., Beisel, B.L., Noireaux, V. Rapid and scalable characterization of CRISPR technologies using an E. coli cell-free transcription-translation system. Mol. Cell 69, 146-157 (2018)

Rustad, M., Eastlund, A., Marshall, R., Jardine, P., Noireaux, V. Synthesis of infectious bacteriophages in an E. coli-based cell-free expression system. J Vis Exp 126, DOI: 10.3791/56144 (2017)

Majumder, S., Garamella, J, Noireaux, V., Liu, A. Cell-free expression of the mechano-sensitive E. coli membrane protein MscL in a synthetic cell system. Chem Comm 53(53), 7349-7352 (2017)

Tayar, A.M., Karzbrun, E., Noireaux, V., Bar-Ziv, R.H. Synchrony and pattern formation of coupled genetic oscillators on a chip of artificial cells. Proc. Nat. Acad. Sci. USA 114, 11609-11614 (2017)

Garamella, J., Marshall, R., Rustad, M., Caschera, F., Noireaux, V. The all E. coli Cell-free TX-TL Toolbox 2.0: a platform for cell-free synthetic biology. ACS Synthetic Biology DOI: 10.1021/acssynbio.5b00296 (2016)

Tayar, A., Karzbrun, E., Noireaux, V., Bar-Ziv, B.H. Propagating gene expression front in a one-dimensional coupled system of artificial cells. Nature Physics 11, 1037-1041 (2015)

Chemla, Y., Ozer, E., Schlesinger, O., Noireaux, V., Alfonta, L. Genetically expanded cell-free protein synthesis using endogenous pyrrolysyl orthogonal translation system. Biotechnol Bioeng 112(8), 1663-1672 (2015)

Caschera, F., Noireaux, V. Integration of biological parts towards the synthesis of a minimal cell. Curr Opin Chem Biol 22, 85-91 (2014)

Karzbrun, E, Tayar, A., Noireaux, V., Bar-Ziv, B.H. Programmable, two-dimensional DNA compartments as artificial cells. Science 345(6198) 829-832 (2014).