NMR Instruments

Video: Mini-lab tour for undergraduate organic labs

Our laboratory maintains five nuclear magnetic resonance (NMR) spectrometers.

Bruker Avance III HD 500 MHz (HD-500)

This Bruker Avance III HD 500 MHz is a 2-channel spectrometer with a shielded Ascend magnet and a 60-place SampleXpress autosampler. There is one probe available, a 5 mm Prodigy TCI-H&F cryoprobe (single axis Z-gradient). This instrument is typically run under full automation with IconNMR. The magnet sits on a Technical Manufacturing Corporation vibration table to protect it from light rail vibrations. The magnet and autosampler were installed in 2011, and the console and Prodigy probe were installed in July 2013. It is located in 194 Kolthoff Hall.

5 mm Prodigy TCI-H&F cryoprobe specifications (¹H or ¹⁹F on the inner coil, ¹³C on an outer coil):

• ¹H S/N: 2500:1
• ¹³C S/N: 350:1
• ¹⁹F S/N: 2000:1
• Temperature range: this spectrometer is not typically used for variable temperature work and it is normally set to 298 K.  However, the probe is capable of 0 °C to 135 °C.

Publication acknowledgement statement for Bruker 500 MHz spectrometers: Research reported in this publication was supported by the Office of the Director, National Institutes of Health, under Award Number S10OD011952. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Bruker Avance III 500 MHz (AV-500)

This Bruker Avance III 500 MHz is a three-channel spectrometer with a shielded Ascend magnet and a 24-place SampleCase autosampler. Two probes are available. The typically installed probe is a 5 mm BBFO SmartProbe (single axis Z-gradient); it has two channels: broadband (BB) and ¹H. The second available probe is a 5 mm TBO triple resonance broadband probe (single axis Z-gradient); it has three channels: BB, ¹H, and ¹⁹F.  The TBO probe allows acquisition of ¹³C spectra with simultaneous ¹H and ¹⁹F decoupling: ¹³C{¹H+¹⁹F}. Please contact NMR personnel if you desire a probe change. A BCU-1 chiller is available for decreasing the probe temperature to ~0 °C.  Temperatures below 0 °C are accessible through the use of a liquid nitrogen evaporator accessory.  This instrument is run in manual mode, but automation is available with the autosampler through IconNMR. The console and probes were installed in December 2011, and magnet and samplechanger were installed in 2013. It is located in 196 Kolthoff Hall.

5 mm BBFO SmartProbe specifications (BB on inner coil, ¹H on outer coil):

• ¹H S/N: 730:1
• ¹³C S/N: 250:1
• ¹⁹F S/N: 550:1
• ³¹P S/N: 180:1
• Temperature range: -150 °C to +150 °C
• Currently available nuclei: ¹H, ²H, ⁷Li, ¹¹B, ¹³C, ¹⁵N, ¹⁷O, ¹⁹F, ²³Na, ²⁷Al, ²⁹Si, ³¹P, ³³S, ⁵¹V, ⁷⁷Se, ⁹⁵Mo, ¹¹⁹Sn, ¹⁹⁵Pt.  Other nuclei can be made available upon request.

5 mm TBO triple resonance broadband probe specifications (BB on inner coil, ¹H and ¹⁹F on an outer coil):

• ¹H S/N: 280:1
• ¹³C S/N: 220:1
• ¹⁹F S/N: 220:1
• ³¹P S/N: 140:1
• Temperature range: -150 °C to +150 °C
• Currently available nuclei: ¹H, ²H, ¹¹B, ¹³C, ¹⁵N, ¹⁷O, ¹⁹F, ²⁷Al, ²⁹Si, ³¹P, ¹¹⁹Sn, ¹²⁷I.  Other nuclei can be made available upon request.

Publication acknowledgement statement for Bruker 500 MHz spectrometers: Research reported in this publication was supported by the Office of the Director, National Institutes of Health, under Award Number S10OD011952. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Bruker Avance III HD 400 MHz (AM-400)

This Bruker Avance III HD 400 MHz nanobay is a 2-channel spectrometer with a shielded Ascend magnet. There is only one probe available, a 5 mm BBFO SmartProbe (single axis Z-gradient). A liquid nitrogen evaporator accessory is available for low-temperature work. It was installed in May 2015, and is located in 194 Kolthoff Hall. 

5 mm BBFO SmartProbe Specifications (BB on inner coil, ¹H on outer coil):

• ¹H S/N: 480:1
• ¹³C S/N: 200:1
• ¹⁹F S/N: 500:1
• ³¹P S/N: 150:1
• Temperature range: -150 °C to +150 °C
• Currently available nuclei: ¹H, ²H, ⁷Li, ¹¹B, ¹³C, ¹⁵N, ¹⁹F, ²³Na, ²⁷Al, ²⁹Si, ³¹P, ⁷⁷Se, ⁹⁵Mo, ¹¹⁹Sn, ¹⁹⁵Pt.  Other nuclei can be made available upon request.

Acknowledgement statement for Bruker 400 MHz spectrometers: Research reported in this publication was supported by the Office of the Vice President of Research, College of Science and Engineering, and the Department of Chemistry at the University of Minnesota.

Bruker Avance III HD 400 MHz (AX-400)

This Bruker Avance III HD 400 MHz nanobay is a two-channel spectrometer with a shielded Ascend magnet and 60-place SampleXpress autosampler. This instrument is running under full automation with IconNMR. There is only one probe available, a 5 mm BBFO SmartProbe (single axis Z-gradient). It was installed in May 2015, and is located in 194 Kolthoff Hall. 

5 mm BBFO SmartProbe Specifications (BB on inner coil, ¹H on outer coil):

• ¹H S/N: 480:1
• ¹³C S/N: 200:1
• ¹⁹F S/N: 500:1
• ³¹P S/N: 150:1
• Temperature range: this spectrometer is not typically used for variable temperature work and it is normally set to 298 K.  However, the probe is capable of -150 °C to +150 °C.
• Currently available nuclei: ¹H, ¹³C, ¹⁵N (HSQC and HMBC only), ¹⁹F, and ³¹P.

Acknowledgement statement for Bruker 400 MHz spectrometers: Research reported in this publication was supported by the Office of the Vice President of Research, College of Science and Engineering, and the Department of Chemistry at the University of Minnesota.

Bruker Avance Neo 400 MHz (NC-400)

The Bruker Avance Neo 400 MHz nanobay is a two-channel spectrometer with an Ultrashield 400 magnet (circa 2006) and a SampleCase Plus 60-place autosampler. There is one probe available, a 5 mm BBFO iProbe (single axis Z-gradient).  This spectrometer was purchased for undergraduate teaching use and it is run in full automation with IconNMR.  It was installed in September 2020 and is located in 196 Kolthoff Hall. 

5 mm BBFO iProbe specifications:

• ¹H S/N: 550:1
• ¹³C S/N: 220:1
• ¹⁹F S/N: 550:1
• ³¹P S/N: 200:1
• Temperature range: this spectrometer is not typically used for variable temperature work and it is normally set to 298 K.  However, the probe is capable of -150 °C to +150 °C.
• Currently available nuclei: ¹H, ²H, ¹³C, ¹⁵N, ¹⁹F, ³¹P, and ⁹⁵Mo.  Other nuclei are available upon request.